Synthesis, Characterization and Application of MCM-41@LDH as a New Support for Lysozyme: Central Composite Design to Evaluate Experimental Variables

A precipitation method was used to synthesize MCM-41. Then, the obtained mesoporous modified using layered double hydroxide (MCM-41@LDH). The novel MCM-41@LDH successfully applied for adsorption of lysozyme (LYS) at different conditions such as adsorbent amount, pH solution, stirrer time, and concentration protein which were designed central composite design (CCD). Furthermore, chief characteristics new identified X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) energy dispersive (EDX) analyses. maximum LYS, predicted by CCD 0.025 g support, LYS 300 mg L-1, 7.50, time 55 min. isotherm, kinetic, thermodynamic equations on surveyed. It established that Freundlich isotherm (R2=0.997) second-order kinetic best data. Gibbs free (ΔG°), enthalpy (ΔH°), entropy (ΔS°) values -17334.5 kJ/mol K (at 298.15 K), -17346.3, -0.04 kJ/mol, respectively. Based achieved results including high intensity support (Freundlich constant, n=2.46) negative value ΔG˚ (spontaneity process), it suggests should be a favorable candidate LYS-chromatography separation applications.